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I worked on some medical image processing research two decades ago and I used ImageJ then. I started to pick up some medical image processing projects again (some registration and segmentation). I checked out Fiji, which seems to be a successor of ImageJ. But the project does not seem to be very active anymore. I wonder if anyone what is a good alternative platform? I am mostly a Python programmer now and am familiar with machine learning backends like PyTorch and Keras.

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I used to recommend ICY from Institut Pasteur, mostly dedicated to biomedical image analysis, it was active in June 2022, and CImg Library - C++ Template Image Processing Toolkit, a small and open-source C++ library for image processing, promoted by dear colleague David Tschumperlé (G'MIC twitter)(lastest version 3.1.4).

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If you’re looking for a large collection of image processing functionality geared towards quantification, I will recommend DIPlib (I’m an author). It is different from ImageJ in that it doesn’t have a GUI, you need to write code. It is a C++ library that you can also use from within Python. It is useful for any imaging modality and application.

If you rather build an image processing pipeline through a GUI, I will recommend CellProfiler. This one is aimed specifically at cell microscopy, though I guess you could use it also for other applications.

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I just learned about ModularImageAnalysis for ImageJ/FIJI. I am linking the paper about it, too.

https://mianalysis.github.io/index.html

https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13227

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