Yes these are the same in practice; you can do the same operations on the real ADC samples. The FFT of a set of samples from the ADC will be a complex result consisting of positive frequencies for the lower half of the result and negative frequencies for the upper half of the result. The real signal that the ADC in sampling have a Nyquist range in frequency from $-f_s/2$ to $+f_s/2$ (where $f_s$ is the sampling rate), which the FFT output is representing. For real signals, such a spectrum is complex conjugate symmetric (Hermitian symmetric), so the negative frequencies are redundant and therefore can be discarded. However, and beyond the detail of this post, we can instead have two real ADC's for which we can sample complex signals in which case the positive and negative frequencies can be completely independent. This exists in practice.
It's all equally abstractions: Real numbers, imaginary numbers, complex numbers, positive frequencies, negative frequencies. How can we say one "exists" and others don't? Because we use two real numbers (on paper) to describe a single complex number, does that mean the complex number is somehow less of a "reality" than a real number? It is the same thing in implementation. We implement complex data paths routinely in the construction of modern radio hardware. We just need two data paths to do such an implementation. In the same fashion, negative frequencies "exist", just as much as positive frequencies do. These are our tools to describe the physical world.
A common sinusoid, which we are first introduced to and therefore so comfortable with as being "reality" is composed of a positive and negative frequency given Euler's identity:
$$\cos (\omega t) = \frac{1}{2}e^{j \omega t} + \frac{1}{2}e^{-j \omega t}$$
Now from that, hopefully we have some clarity on what exactly a positive or negative frequency is (and that it "exists"!). A sinusoid itself does not represent a positive or negative frequency, but consists of both. A positive or negative frequency is specifically of the form of $e^{j \omega t}$ where the sign of the exponent indicates if this is a positive or negative frequency. Since $e^{j\phi}$ is simply a phasor with magnitude $1$ and angle $\phi$, a positive frequency is represented in the time domain as a complex phasor rotating counter-clockwise, and negative frequency is represented in the time domain as a complex phasor rotating clockwise. Any complex phasor rotating in the time domain at a constant rate with constant magnitude will be an impulse in the frequency domain.
After answering I realized this was asked and answered before, please see: Does negative frequency actually exist or it is just theoretical?
Please also see my favorite question here related to this (use of negative frequencies and specifically complex numbers to describe physical signals). So as long as we have two scope probes we can measure actual signals that are not real, but composed of pairs of real numbers (complex!), and so to the same extent a real signal "exists in practice", so too does a complex signal. Under the same definition of "existence", which is applicable to the question if negative frequencies exist in practice, I say yes they do!