As known, the pilot tones are transmitted via subcarriers predefined and known in the transmitter and the receiver. The pilot spacing is repeated with a certain rate that depends on how fast the channel changes.
I am talking about comb-type channel estimation. The distance between every two pilot's sub-carrier is called, here, pilot's spacing.
If we have number of sub-carriers $N$ = 64; in the first case let's take 8 sub-carriers to transmit pilot's tones defined in locations 1,9,17...; with pilot's spacing $j_1 = \frac{64}{8} = 8$. The second case let's take 16 sub-carriers defined in locations 1,5,9,13...; with pilot's spacing $j_2 =\frac{64}{16} = 4$.
It's clear that second case pilots can track the time-varied channel better. But, with an OFDM system, the symbol data is converted into time domain using $iFFT$, and every subcarrier is spread over all the other sub-carriers including pilot's sub-carriers. How does the pilot's spacing affect the performance?