I have a signal that I measured by optical means using a focused laser. The laser scans over a microscopic feature (in step size of 0.33 micron) and I have plotted the result of the measurement as a function of distance (x scale is microns):
I am interested in the width of the feature I am measuring, however in this case the width seems on the same order (or even much smaller) than the laser spot size. This results in the signal being a Gaussian shape which could potentially just be the shape of the laser spot. But I cannot know that for sure, because I'm trying to measure the width of this feature. It is entirely plausible that the feature itself gives a Gaussian response, I expect it is not an extremely sharp edge. But I'm trying to determine how sharp the edge is exactly, and the laser spot size is now limiting.
Assuming the feature is extremely thin and sharp, then the measurement would indeed yield this Gaussian shape. However the feature is definitely not infinitely sharp and I believe I can measure more accurately what its shape is by using deconvolution.
I have tried to measure the laser spot size (this would be the system response function, I guess?) by scanning over a feature that IS definitely 100% sharp. If everything was perfect the signal from that would be a step function, but of course due to the large laser spot size I actually get a sort of error function shape from which I believe I can determine the laser spot size:
Sharp edge signal image:
Unless I'm mistaken the parameter C in my fit gives me the 1/e^2 beam width (0.785 micron). I think I can use this to form the system response function, assuming that my laser beam is Gaussian (which is probably not true but that's the best I can do).
The original signal I measured should be a convolution of the actual feature and this Gaussian system response, so I should also be able to deconvolve the signal with this same Gaussian system response and therefore obtain a better measurement of the feature, right?
How would I go about doing this? I have the original signal data (image 1, distance vs signal) and I can assume a Gaussian spot for my laser as the system response function with the known size obtained from the measurement of the perfectly sharp edge (image 2), as far as I understand (not much more than this...) this should be enough to do a deconvolution?