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Is there any way to use as single input an image (256x256 pixels) and get output of single value using Matlab neural network toolbox

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Sure, import the image to the matlab workspace, divide the image matrix into two dimentional matrices like this:

matrix1 = yourimage(:,:,1);
matrix2 = yourimage(:,:,2);
matrix3 = yourimage(:,:,3);

each being one colour component-as nntool works with up to bidimensional matrices- and use them in the nntool.

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